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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: J Virol Methods. 2010 May 13;168(1-2):155–161. doi: 10.1016/j.jviromet.2010.05.008

Figure 3. Recognition of gp120 variants by CD4 and monoclonal antibodies.

Figure 3

(A) Comparable amounts of radiolabeled wild-type (WT) and mutant gp120 glycoproteins in transfected 293T cell supernatants were incubated with a polyclonal mixture of sera from HIV-1-infected individuals (PS), CD4-Ig, or different monoclonal antibodies (13 nM) for two hours at 37°C. Precipitates were analyzed by SDS-PAGE without β-mercaptoethanol followed by autoradiography/ densitometry. The results shown are representative of those obtained in two independent experiments. The gp120 dimer is designated gp120-D. (B) The gp120 bands observed in (A) were quantified by densitometry. Results are expressed as the percentage of dimeric gp120 relative to the total amount of gp120 (monomers + dimers). Data shown represent the means +/- SEM of two independent experiments.