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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: J Virol Methods. 2010 May 13;168(1-2):155–161. doi: 10.1016/j.jviromet.2010.05.008

Figure 5. Effect of deletion of the gp120 N/C-termini and variable loops on dimer formation.

Figure 5

293T cells were transfected with plasmids expressing wild-type (wt) HIV-1YU2 gp120, the 44-492 gp120, or gp120 protein with deletions of the V1/V2 and/or V3 variable loops. Comparable amounts of radiolabeled wild-type (wt) and mutant gp120 glycoproteins in transfected 293T cell supernatants were incubated with a polyclonal mixture of sera from HIV-1-infected individuals for two hours at 37°C. Precipitates were analyzed by SDS-PAGE without β-mercaptoethanol followed by autoradiography/ densitometry. The results shown are representative of those obtained in four independent experiments and are normalized to the amount of dimer observed for wt gp120.