Figure 7.
Proliferative response and cytokine productions of α-turmerone (AL) and ar-turmerone (AR) treated PBMC. (A) PHA-activated PBMC were seeded in 96-well plates and incubated with 5, 10 or 15 μg/ml of α-turmerone (AL) or ar-turmerone (AR) in culture medium for 72h, and expressed as the mean % ratio of count per minute in treated and untreated control cells of 8 individual blood donors. The resting PBMC (B, D, E) and PHA-activated PBMC (C) were seeded in 96-well plates and incubated with 5, 10 or 15 μg/ml of α-turmerone (AL) or ar-turmerone (AR) for 24 hours. Culture supernatants were collected and the cytokine concentrations were specifically determined by ELISA. Results were expressed as mean concentration + SD of 8 blood samples with four wells each. Differences between the treated and untreated control group were determined by Student’s unpaired t-test. * P < 0.05, **P < 0.01 as compared to the control group.