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. Author manuscript; available in PMC: 2011 Jul 1.

Published in final edited form as: Photochem Photobiol. 2010 May 21;86(4):781–791. doi: 10.1111/j.1751-1097.2010.00750.x

Hydroperoxide formation in human RPE cells pretreated with all-trans-retinal (atRal) or A2E and exposed to visible light. The cells were incubated in the dark in medium containing 25 and 50 μM atRal or 12.5 and 25 μM A2E for 5 hours. Then the medium was replaced with PBS and the cells were exposed to light (λ>400nm) for 5 and 20 min. After irradiation the cells were collected and frozen. Total hydroperoxide concentration (a) and the level of lipid hydroperoxides (b) in the cells were determined with the FOX assay and normalized to protein concentration in hRPE cells treated in the same way.

Hydroperoxide formation in human RPE cells pretreated with all-trans-retinal (atRal) or A2E and exposed to visible light. The cells were incubated in the dark in medium containing 25 and 50 μM atRal or 12.5 and 25 μM A2E for 5 hours. Then the medium was replaced with PBS and the cells were exposed to light (λ>400nm) for 5 and 20 min. After irradiation the cells were collected and frozen. Total hydroperoxide concentration (a) and the level of lipid hydroperoxides (b) in the cells were determined with the FOX assay and normalized to protein concentration in hRPE cells treated in the same way.