Table 1.
Gene | Sequence | Amplicon Length | Cycles |
---|---|---|---|
MMP-2 | 5′ – agatcttcttcttcaaggaccggtt– 3′ (sense) 5′ – ggctggtcagtggcttggggta– 3′ (antisense) |
225 | 35 |
MMP-9 | 5′ – gtttttgatgctattgctgagatcca– 3′ (sense) 5′ – cccacatttgacgtccagagaagaa3′ (antisense) |
208 | 35 |
TIMP-1 | 5′ – ggcatcctcttgttgctatcactg– 3′ (sense) 5′ – gtcatcttgatctcatcccgctgg– 3′ (antisense) |
169 | 35 |
TIMP-2 | 5′ – ctcgctggacgttggaggaaagaa– 3′ (sense) 5′ – agcccatctggtacctgtggttca– 3′ (antisense) |
155 | 35 |
HIF-1α | 5′ –agtgatgaaagaattact– 3′ (sense) 5′ –aataataccacttacaaca– 3′ (antisense) |
2759 | 35 |
VEGF-A | 5′ –atgaagtgatcaagttcatgg– 3′ (sense) 5′ –ggatcttggacaaacaaatgc– 3′ (antisense) |
360 | 35 |
VEGFR-1 | 5′ –tttccatttgatactcttac– 3′ (sense) 5′ –tcttagttgctttaccaggg– 3′ (antisense) |
310 | 35 |
VEGFR-2 | 5′ –tgtggttgtaggatataggat– 3′ (sense) 5′ –aaaggctttgtgtgaactcgg– 3′ (antisense) |
338 | 35 |
ADAMTS-1 | 5′ –cattaacggacaccctgctt– 3′ (sense) 5′ –cgtgggacacacatttcaag– 3′ (antisense) |
166 | 35 |
18S | 5′ –agctaggaataatggaatag– 3′ (sense) 5′-aatcaagaacgaaagtcggag- 3′ (antisense) |
150 | 19 |