Expression of Akt, pAkt, AMPK and pACC in myocardium from young or old wild-type (WT) and AMPK deficient KD mice. A: Akt; B: pAkt; C: AMPK; and D: pACC (ser79). Inset: Representative gel blots of Akt, pAkt, AMPK and pACC using specific antibodies. GAPDH was used as the loading control. Mean ± SEM, n = 3 – 6 hearts per group, *p – 0.05 vs. respective young group, #p – 0.05 vs. WT-old group.