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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Aging Cell. 2010 May 10;9(4):580–591. doi: 10.1111/j.1474-9726.2010.00585.x

Fig. 2. shRNA-mediated knockdown of WRNp in NCCIT cells.

Fig. 2

NCCIT cells were transfected with plasmids encoding one of two types of shRNA against WRN, or shRNA against G9a, or one of two control plasmids (CV – empty vector, CS – vector control expressing a scrambled, non-targeting sequence, G(−) – G9a-deficient clone, 31–34, 61, and 62 – WRNp deficient clones, see Supplementary Table 3). Transfected cells were selected in puromycin and individual clones were isolated and subjected to western blotting analysis with a WRNp antibody or a γ-tubulin control.