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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Aging Cell. 2010 May 10;9(4):580–591. doi: 10.1111/j.1474-9726.2010.00585.x

Fig. 3. WRNp knockdown does not affect changes in histone methylation during pluripotent cell differentiation.

Fig. 3

Q-ChIP analysis of Oct4 promoter DNA associated with H3K4me2 (dimethylated lysine 4 of H3) and H3K9me3 (trimethylated lysine 9 of H3) in undifferentiated and RA treated cells. Results are presented as a ratio of amplicons (Oct4 B primer/probe set, see Supplementary Table 2) detected in differentiated over non-differentiated samples. Cells were treated with 10 µM RA for 72 hrs, when histone methylation changes were shown to peak (Feldman et al. 2006).