A–F, Expression of DA markers in the ventral midbrain of adult Etv5−/− mice. Coronal sections of the brains of adult control (WT, A–C) and Etv5−/− mice (KO, D–F) were examined by immunofluorescence for the expression of TH and DAT. These markers are highly coexpressed in the DA neurons and their fibers, and are unchanged in the Etv5 knock-out. Inset boxes in A and D show the location of the enlarged areas in B, C and E, F. Scale bars: (in D) A, D, 500 μm; (in E) B, C, E, F, 250 μm.