Table 4.
Adaptor Ligation Master Mix (per sample):
| Component | Volume |
|---|---|
| Annealed paired-end adaptor (50μM) | 5 μl |
| 10X T4 Ligation Buffer | 5 μl |
| T4 ligase (400 U/μL) | 5 μl |
| TOTAL | 15 μl |
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