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Master mix for Pre-array bulk Amplification (per sample)
| Number of reactions (23 DNA, 1 no template control) | 24 |
| Total volume with DNA template | 1200 μl |
| Component | Volume |
| Water | 823.2 μl |
| 5x Phusion buffer (5x → 1x) | 240 μl |
| 25mM dNTPs [25mM → 200μM] | 9.6 μl |
| EXOME_BULK_ FWD [100μM → 500nM] | 6 μl |
| EXOME_BULK_ REV [100μM → 500nM] | 6 μl |
| Phusion HotStart (2U/μl → 0.02U/μl) | 12 μl |
| TOTAL | 1100 μl |
