Table 8.
Master mix for Pre-sequencing Amplification (per sample)
Component | Volume |
---|---|
Water | 3 μL |
2x iTaq Supermix | 25 μL |
EXOME_SEQ_ FWD [10μM →200nM] | 1 μL |
EXOME_SEQ_ REV [10μM →200nM] | 1 μL |
TOTAL | 30 μL |
Master mix for Pre-sequencing Amplification (per sample)
Component | Volume |
---|---|
Water | 3 μL |
2x iTaq Supermix | 25 μL |
EXOME_SEQ_ FWD [10μM →200nM] | 1 μL |
EXOME_SEQ_ REV [10μM →200nM] | 1 μL |
TOTAL | 30 μL |