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. 2010 Jun 4;29(14):2407–2420. doi: 10.1038/emboj.2010.112

Figure 1.

Figure 1

Mcl-1 protein levels are regulated through the cell cycle. (A) Mcl-1 levels increase from G1 through to mitosis. Human U2OS cells were synchronised at the G1/S boundary by double thymidine block then released into the cell cycle. Samples were analysed by SDS–PAGE and immunoblotting using the specified antibodies at the times shown after release from the block. The predominant cell cycle phase at each time was confirmed by FACS analysis in parallel experiments (data not shown) as in Bennett and Clarke (2006). (B) Mcl-1 protein levels decrease in response to anti-mitotic drugs. Human U2OS cells growing in asynchronous (As) culture were treated with 200 ng/ml nocodazole (Noc) or 0.5 μM taxol for 8 or 24 h. Floating, mitotic cells (M) were separated from adherent, interphase cells (A) at each time and samples were analysed by immunoblotting using the specified antibodies.