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Effect of ThPOK deficiency on peripheral γδ T cell subsets. (A) FACS analysis of total or gated γδTCR+ splenocytes, as indicated, from HD/HD, HD/+ and ThPOKconst mice, showing expression of NK1.1, CCR6 and DRE-GFP reporter. (B) FACS analysis of γδTCR+ splenocytes, showing Vγ2 expression for indicated gated NK1.1/CCR6 subsets. (C) Absolute numbers of indicated γδTCR+ splenic subsets from 1-month-old HD/HD (n=4), HD/+ (n=5) and ThPOKconst mice (n=5). Error bars indicate s.d.
