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. 2010 Jun 8;29(14):2446–2460. doi: 10.1038/emboj.2010.116

Figure 3.

Figure 3

Maxer is a novel ER protein. (A) Confocal images show colocalization of Maxer and ER-DsRed in HeLa cells. Higher magnifications are shown from the fourth to sixth lines. Maxer 1–711 is colocalized with ER-DsRed, whereas Maxer 1–683 showed diffuse distribution. Single transfection of Maxer or ER-DsRed did not make signals that bleed through fluorescence. Bar: 10 or 1 μm in higher magnification. (B) Deletion constructs of Maxer–EGFP (left). Maxer and Maxer 1–711 were enriched in cellular membrane fraction of HeLa cells, whereas Maxer 1–683 was enriched in cytosolic fraction (right); α-tubulin and calnexin were used as cytosolic and membrane fraction marker, respectively. (C) Density gradient fractionation showed Maxer enrichment in ER, but not golgi or mitochondria. Calnexin (ER), golgi 58 K (golgi) and Tom70 (mitochondria) were used as organelle markers. (D) Confocal images showed colocalization of endogenous Maxer and ER-DsRed. Bar: 10 μm.