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. 2010 Jun 8;29(14):2446–2460. doi: 10.1038/emboj.2010.116

Figure 6.

Figure 6

Reduction of Bergmann glia and GLAST in Atx1-KI mice. (A) Bergmann glia were reduced in the cerebellum of mutant Atx1-KI mice. Bergmann glia and Purkinje cells were immunostained with anti-Sox2 antibody and anti-calbindin antibody, respectively. Corresponding cerebellar folia from three different ages (1, 16 and 40 weeks) were analysed. Bar: 50 μm. (B) Quantification of Bergman glia number (left) and Purkinje cell number (right) of three different ages (1, 16 and 40 weeks). Error bar: s.e.m. n=4, *P<0.01, Student's t-test. (C) Western blot analysis with anti-Sox2 antibody to quantify Bergmann glia. The ratio indicates GAPDH-corrected signal intensity of Sox2. (D) Mutant Atx1 expression reduced GLAST in KI mice. Cerebella from WT and Atx1-KI were stained with anti-GLAST antibody (upper). GLAST signal intensity was quantified (lower). The error bars represent s.e.m. n=4 mice, *P<0.01, Student's t-test. Bar: 50 μm. (E) Western blot analysis of GLAST expression in WT and Atx1-KI cerebellum.