Table 1.

Antigen	Primary	Dilution	Antibody	Secondary	Specificity &
	antibodies/		Source	antibodies/antisera	Subcellular distribution
	antisera			(dilutions)
Estrogen	Mouse	1:10	Chemicon	Goat anti-mouse IgG	Neurons and astrocytes /
receptor	IgG1		(MAB428)	(H+L)conjugated to Cy3	Punctate nuclear and
(ER)	monoclonal			(1:250)	perinuclear distribution
Dopamine	Rabbit	1:1000	Chemicon	• Goat anti-rabbit IgG	Neuronal processes and cell
(DA)	IgG		(AB122S)	DTAF (1:500) or	body
	polyclonal			• Goat anti-rabbit IgG	Cytoplasmic distribution
				Cy3 (1:250)
Dopamine	Rat	1:100	Chemicon	Goat anti-rat IgG	Neuronal processes and cell
transporter	Ig type		(MAB369)	conjugated to biotin-	body; astrocytes
(DAT)	monoclonal			avidin-Cy2 (1:250)	Cell membrane
Dopamine	Rabbit	1:100	Chemicon	Goat anti-rabbit IgG	Neurons and astrocytes;
D1A	polyclonal		(AB1784P)	DTAF (1:500)	punctate distribution
receptor
Dopamine	Rabbit	1:500	Chemicon	Goat anti rabbit IgG	Neuronal processes and cell
D2	polyclonal		(AB1558)	DTAF (1:500)	bodies: perinuclear
receptor					distribution, astrocytes,
					microglia
Co-localization
Combined				• Goat anti-rabbit IgG	Colocalized in neurons
• DA +	as above	as above	as above	DTAF +	and/or astrocytes
• ER				• Goat anti-mouse
				Cy3
Combined				• Goat anti-rabbit IgG	Colocalized in
• DA +	as above	as above	as above	Cy3	subpopulations of neurons
• DAT				• Goat anti-rat IgG	and/or astrocytes
				biotin-avidin Cy2
Combined	as above	as above	as above	• Goat anti-rabbit IgG	Colocalized in
• D1A +				DTAF (1:500)	subpopulations of neurons
• ER				• Goat anti-mouse	and/or astrocytes
				Cy3 (1:250)
Combined	as above	as above	as above	• Goat anti-rabbit IgG	Colocalized in
• D2 +				DTAF (1:500)	subpopulations of neurons
• ER				• Goat anti-mouse	and/or astrocytes and
				Cy3 (1:250)	microglia