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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: J Immunol. 2010 Apr 19;184(10):5502–5509. doi: 10.4049/jimmunol.0901601

Figure 1. SMAD4 participates in endotoxin tolerance development.

Figure 1

A. THP1 cells were treated with 100ng/ml or 10-100ng/ml of LPS for 0, 1, 3, 6 and 24 hours. After a specified period, cells were harvested for Western blot analysis and culture supernatants assessed for TNF-α concentration. Each treatment was executed in triplicates. Results are shown as mean ± SD. B. Cytosolic and nuclear proteins were isolated from the above treated cells and subjected to Western blot analysis with indicated antibodies. All blots were reprobed with anti-β-Actin to show equal loading. All results are representative of three separate experiments. C. THP1 cells were treated with 100ng/ml or 10-100ng/ml of LPS for 0, 2 and 10 min. After a specified period, cells were harvested for Western blot analysis against p-SMAD2, SMAD2 or -β-Actin.