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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: J Immunol. 2010 Apr 19;184(10):5502–5509. doi: 10.4049/jimmunol.0901601

Figure 8. SHIP1 regulates IRAK-M expression during endotoxin tolerance development in THP1 cells.

Figure 8

A. shSHIP1 and shLUC THP1 cells were treated with 100ng/ml or 10-100ng/ml of LPS for 0, 3, 6 and 24 hours. After indicated periods, cells were harvested for Western analysis with indicated antibodies. The blots were reprobed with anti-β-Actin to show equal loading. B. shSHIP1 and shLUC THP1 cells were grown in serum free media overnight and stimulated with TGF-β1 (10ng/ml) for 0, 3 6 and 24 hours. Cell lysates were subjected to Western analysis with indicated antibodies. Blots were reprobed with anti-β-Actin to show equal loading. shSHIP1: shSHIP1 RNA; shLUC: control shRNA. Each experiment was repeated at least three times. Each experiment was repeated at least three times.