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. Author manuscript; available in PMC: 2011 Sep 15.
Published in final edited form as: J Comp Neurol. 2010 Sep 15;518(18):3645–3661. doi: 10.1002/cne.22445

Figure 7.

Figure 7

Absence of Fgf8 at the commissural plate in the Emx1-cre;Fgf8flox/flox mice. A: Immunohistochemistry shows EMX1 expression in the pallium and medial telencephalon extending ventrally to the lamina terminalis. B: Emx1-cre and ROSA26flox/flox mice were crossed to obtain a green fluorescent protein (GFP) profile of the extent of Emx1-cre recombinase activity. Immunohistochemistry for GFP at E14 reveals recombinase activity in the pallium. In particular, recombinase activity occurs around the dorsal part of the lamina terminalis (arrow). C and D: In situ hybridization in Emx1+/+;Fgf8flox/flox (Ctl) mice demonstrates that Fgf8 is expressed in the commissural plate at E14 at the border of the MC and SA domains. By contrast, sense in situ hybridization in control mice (E) and antisense in situ hybridization in Emx1-cre;Fgf8flox/flox (cKO) mice (F) reveal no Fgf8 expression. Due to the restricted overlay of expression domains, the telencephalon develops without major deficit until E14. Scale bar = 400 μm.