Fig 6.

Depletion of Twi compromises target expression and Twi is present at target enhancers prior to Notch activation. (A) Fold change in the expression levels of the indicated genes after Notch activation as determined by quantitative RT-PCR in the absence (light grey) or presence (dark grey) of twi RNAi. The average of three biological replicates is shown. (B) Expression of the indicated Notch target genes in wild type and in muscle progenitors expressing twi RNAi. (C,D) Enrichment of fragments from the indicated genes in anti-Twi ChIP from DmD8 cells before Notch activation. T, Twi binding/enhancer region; ORF, open reading frame. Results are the average of three independent experiments; error bars indicate s.e.m.