Table 1.
Strains/plasmids | Relevant characteristics* | Reference or source |
Strains | ||
E. faecium | ||
TX82 | Endocarditis isolate. Vanr, Ampr | 61 |
TX5645 | TX82ΔebpABCfm, ebpABCfm deletion mutant | This study |
TX5664 | TX5645 (pAT392), deletion mutant with empty complementation vector. Genr | This study |
TX5665 | TX5645 (pAT392::ebpABCfm), deletion mutant complemented with ebpABCfm. Genr | This study |
TX5667 | TX82 (pAT392), WT with empty complementation vector. Genr | This study |
TX5668 | TX82 (pAT392::ebpABCfm), WT supplemented with ebpABCfm. Genr | This study |
TX2154 | E1162, blood isolate. Ampr | 27 |
TX2155 | E1162Δesp, deletion mutant. Ampr, Chlr | 27 |
E. coli | ||
DH5α | E. coli cloning host | Invitrogen |
XL1 Blue | E. coli cloning host, Tetr | Stratagene |
TX5644 | DH5α (pTEX5644) | This study |
TX5666 | XL1 Blue (pAT392::ebpABCfm) | This study |
Plasmids | ||
pTEX5501ts | Temperature sensitive plasmid, used for deletion mutagenesis of E. faecium. Chlr, Genr | 61 |
pTEX5644 | Plasmid for ebpABCfm deletion; flanking regions of ebpABCfm cloned into pTEX5501ts. Chlr, Genr | This study |
pAT392 | Complementation vector. Genr | 62 |
pTEX5665 | pAT392::ebpABCfm; plasmid for complementation of the ebpABCfm mutant. Genr | This study |
Van, vancomycin; Amp, ampicillin; Gen, gentamicin; Tet, tetracycline; Chl, chloramphenicol; superscript “s” designates sensitivity, “r” designates resistance.