Skip to main content
. 2010 Jul-Aug;1(4):236–246. doi: 10.4161/viru.1.4.11966

Table 1.

Bacterial strains and plasmids used in this study

Strains/plasmids Relevant characteristics* Reference or source
Strains
E. faecium
TX82 Endocarditis isolate. Vanr, Ampr 61
TX5645 TX82ΔebpABCfm, ebpABCfm deletion mutant This study
TX5664 TX5645 (pAT392), deletion mutant with empty complementation vector. Genr This study
TX5665 TX5645 (pAT392::ebpABCfm), deletion mutant complemented with ebpABCfm. Genr This study
TX5667 TX82 (pAT392), WT with empty complementation vector. Genr This study
TX5668 TX82 (pAT392::ebpABCfm), WT supplemented with ebpABCfm. Genr This study
TX2154 E1162, blood isolate. Ampr 27
TX2155 E1162Δesp, deletion mutant. Ampr, Chlr 27
E. coli
DH5α E. coli cloning host Invitrogen
XL1 Blue E. coli cloning host, Tetr Stratagene
TX5644 DH5α (pTEX5644) This study
TX5666 XL1 Blue (pAT392::ebpABCfm) This study
Plasmids
pTEX5501ts Temperature sensitive plasmid, used for deletion mutagenesis of E. faecium. Chlr, Genr 61
pTEX5644 Plasmid for ebpABCfm deletion; flanking regions of ebpABCfm cloned into pTEX5501ts. Chlr, Genr This study
pAT392 Complementation vector. Genr 62
pTEX5665 pAT392::ebpABCfm; plasmid for complementation of the ebpABCfm mutant. Genr This study
*

Van, vancomycin; Amp, ampicillin; Gen, gentamicin; Tet, tetracycline; Chl, chloramphenicol; superscript “s” designates sensitivity, “r” designates resistance.