Table 4.
Conserved UPEC outer membrane proteins predicted by Vaxign.
| # | Protein RefSeq | Symbol | Adhesin | TMH | Not in K-12 | Micro-array | Prote-omics | Protein Name |
|---|---|---|---|---|---|---|---|---|
| Iron ion binding and iron/siderophore transporter activity | ||||||||
| 1 | NP_752820.1 | ybiL | 0.857 | 1 | − | − | Catecholate siderophore receptor fiu precursor (TonB-dependent receptor fiu) (Ferric iron uptake protein) | |
| 2 | NP_754328.1 | c2436 | 0.473 | 0 | − | − | putative pesticin receptor precursor (tonB-dependent receptor) | |
| 3 | NP_754406.1 | c2518 | 0.83 | 0 | X | − | − | TonB dependent receptor |
| 4 | NP_752238 | c0294 | 0.83 | 0 | X | − | − | TonB dependent receptor |
| 5 | NP_753164.1 | iroN | 0.672 | 0 | + | + | Siderophore receptor iron | |
| 6 | NP_752600.1 | fepA | 0.792 | 0 | + | − | ferrienterobactin receptor (TonB-dependent receptor) | |
| 7 | NP_752135.1 | fhuA | 0.746 | 0 | + | + | ferrichrome outer membrane transporter | |
| 8 | NP_754374.1 | Hma (c2482) | 0.772 | 0 | X | + | + | outer membrane heme acquisition protein |
| 9 | NP_756170 | chuA | 0.846 | 0 | X | + | + | Outer membrane heme/hemoglobin receptor |
| 10 | NP_753551.1 | prrA | 0.589 | 0 | X | − | − | Putative TonB-dependent outer membrane receptor |
| 11 | NP_753179 | c1265 | 0.777 | 0 | X | − | − | Outer membrane heme/ hemoglobin receptor |
| 12 | NP_753125 | c1206 | 0.794 | 0 | X | − | − | Outer membrane heme/haemoglobin receptor |
| 13 | NP_755646 | c3775 | 0.79 | 0 | X | − | − | putative iron compound receptor |
| 14 | NP_753820.1 | yddB | 0.765 | 0 | − | − | hypothetical protein c1924 (tonB-dependent receptor family) | |
| Fimbrial biogenesis outer membrane usher protein | ||||||||
| 15 | NP_757244.1 | fimD | 0.744 | 1 | + | − | Outer membrane usher protein fimD precursor | |
| 16 | NP_757034 | papC_2 | 0.674 | 1 | X | − | − | PapC protein |
| 17 | NP_755465 | papC | 0.666 | 0 | X | − | − | PapC protein |
| 18 | NP_754524.1 | yehB | 0 | 0 | − | − | Outer membrane usher protein yehB precursor | |
| 19 | NP_752120.1 | htrE | 0.643 | 0 | − | − | Putative outer membrane usher protein | |
| 20 | NP_753830.1 | c1934 | 0.856 | 1 | − | − | Outer membrane usher protein fimD precursor | |
| 21 | NP_754765.1 | yfcU | 0.563 | 0 | X | − | − | Fimbrial export usher family protein |
| 22 | NP_753156.1 | focD | 0.854 | 0 | − | − | F1C fimbrial usher | |
| 23 | NP_756076.1 | ycbS | 0.559 | 1 | − | − | Outer membrane usher protein ycbS precursor | |
| 24 | NP_753159 | focH | 0.917 | 0 | X | − | − | F1C putative fimbrial adhesin precursor |
| Porin and ion transport | ||||||||
| 25 | NP_753469.1 | nmpC | 0.788 | 1 | X | − | + | Outer membrane porin protein nmpC precursor |
| 26 | NP_754644.1 | ompC | 0.688 | 0 | − | + | Outer membrane porin protein C | |
| 27 | NP_756858.1 | lamB | 0.806 | 0 | − | + | maltoporin | |
| 28 | NP_752996.1 | ompF | 0.614 | 0 | − | + | Outer membrane protein F (Porin family) | |
| 29 | NP_754240.1 | c2348 | 0.759 | 0 | X | − | − | Outer membrane porin protein nmpC precursor |
| 30 | NP_752325.1 | phoE | 0.729 | 0 | − | − | Outer membrane phosphoporin protein E | |
| 31 | NP_753724.1 | ompN | 0.751 | 0 | − | − | Outer membrane protein N precursor | |
| 32 | NP_754275.1 | c2383 | 0.597 | 0 | X | − | − | Outer membrane protein N precursor |
| 33 | NP_754771.1 | fadL | 0.871 | 0 | − | + | Long-chain fatty acid outer membrane transporter | |
| 34 | NP_756025.1 | hofQ | 0.186 | 0 | + | − | Outer membrane porin HofQ | |
| Other transport proteins | ||||||||
| 35 | NP_756748.1 | c4894 | 0.81 | 0 | X | − | − | Nucleoside-specific channel-forming protein tsx precursor |
| 36 | NP_756500.1 | c4642 | 0.694 | 0 | − | − | Putative outer membrane Protein yieC | |
| 37 | NP_753669.1 | c1765 | 0.437 | 0 | − | − | Partial putative outer membrane channel protein | |
| 38 | NP_752455.1 | tsx | 0.833 | 0 | − | + | Nucleoside-specific channel-forming protein tsx precursor | |
| Lipoproteins | ||||||||
| 39 | NP_756849.1 | yjbH | 0.651 | 0 | − | − | Lipoprotein yjbH precursor | |
| 40 | NP_755008.1 | yfiB | 0.564 | 0 | − | − | Putative outer membrane lipoprotein | |
| 41 | NP_756936.1 | yjcP | 0.185 | 0 | − | − | Putative outer membrane efflux protein MdtP | |
| 42 | NP_752589.1 | cusC | 0.516 | 0 | − | − | Copper/silver efflux system outer membrane protein CusC | |
| 43 | NP_754925.1 | yfhM | 0.224 | 0 | − | − | Lipoprotein yfhM precursor | |
| 44 | NP_756232.1 | yiaD | 0.526 | 3 | + | − | Putative outer membrane lipoprotein | |
| Other outer membrane proteins | ||||||||
| 45 | NP_752286.1 | c0345 | 0.987 | 1 | X | − | − | ShlA/HecA/FhaA exofamily protein |
| 46 | NP_752352 | eaeH | 0.904 | 1 | X | − | − | Putative adhesin |
| 47 | NP_753126 | c1207 | 0.702 | 0 | X | − | − | Hypothetical protein |
| 48 | NP_753493 | c1585 | 0.526 | 0 | X | − | − | Putative tail component of prophage |
| 49 | NP_754912 | c3030 | 0.71 | 1 | X | − | − | SinI-like protein |
| 50 | NP_756286 | c4424 | 0.99 | 0 | X | − | − | Putative adhesin |
| 51 | NP_752116.1 | yadC | 0.81 | 1 | − | − | Putative fimbrial-like adhesin protein | |
| 52 | NP_752162.1 | yaeT | 0.637 | 0 | + | + | Outer membrane protein assembly factor YaeT | |
| 53 | NP_752163.1 | hlpA | 0.587 | 0 | − | − | Periplasmic chaperone | |
| 54 | NP_752339.1 | yagX | 0.571 | 0 | − | − | Hypothetical protein c0402 | |
| 55 | NP_752642.1 | crcA | 0.739 | 1 | − | − | Palmitoyl transferase for Lipid A | |
| 56 | NP_752830.1 | ompX | 0.818 | 1 | + | + | Outer membrane protein X | |
| 57 | NP_753262.1 | flgK | 0.84 | 0 | − | − | Flagellar hook-associated protein FlgK | |
| 58 | NP_753627.1 | ompW | 0.848 | 0 | − | + | Outer membrane protein W | |
| 59 | NP_753695.1 | ompG | 0.618 | 0 | − | − | Outer membrane protein G precursor | |
| 60 | NP_754014.1 | ydiY | 0.708 | 0 | − | − | Hypothetical protein c2120 | |
| 61 | NP_754081.1 | yeaF | 0.852 | 0 | + | + | MltA-interacting protein precursor | |
| 62 | NP_754523.1 | yehA | 0.847 | 0 | − | − | Hypothetical protein c2635 | |
| 63 | NP_754661.1 | yfaL | 0.949 | 0 | − | − | Adhesin | |
| 64 | NP_755530.1 | c3655 | 0.962 | 0 | − | + | Antigen 43 precursor | |
| 65 | NP_756601.1 | pldA | 0.756 | 0 | − | − | Phospholipase A | |
| 66 | NP_754578.1 | cirA | 0.51 | 0 | − | − | Colicin I receptor | |
| 67 | NP_755652.2 | tolC | 0 | 0 | − | + | Outer membrane channel protein | |
| 68 | NP_752583.1 | ompT | 0 | 0 | + | + | Outer membrane protease | |
| 69 | NP_756783.1 | btuB | 0 | 0 | − | + | Vitamin B12/cobalamin outer membrane transporter | |
| 70 | NP_754246.1 | fliF | 0.213 | 2 | − | − | Flagellar MS-ring protein | |
| 71 | NP_755960.1 | yheF | 0.239 | 0 | − | − | General secretion pathway protein D precursor | |
| 72 | NP_752585.1 | nfrA | 0.298 | 0 | − | − | Bacteriophage N4 receptor, outer membrane subunit | |
| 73 | NP_753902.1 | uidC | 0.304 | 1 | − | − | Putative outer membrane porin protein | |
| 74 | NP_754656.1 | c2770 | 0.348 | 0 | − | − | Hypothetical protein c2770 | |
| 75 | NP_757165.1 | ytfM | 0.4 | 0 | − | − | Hypothetical protein c5318 | |
| 76 | NP_753730.1 | ydbH | 0.401 | 1 | − | − | Hypothetical protein c1828 | |
| 77 | NP_756204.1 | yhjL | 0.408 | 0 | − | − | Cellulose synthase subunit BcsC | |
| 78 | NP_751977.1 | c0021 | 0.418 | 1 | − | − | Hypothetical protein c0021 | |
| 79 | NP_757166.1 | ytfN | 0.426 | 1 | − | − | Hypothetical protein c5319 | |
| 80 | NP_754559.2 | yohG | 0.427 | 0 | + | − | Multidrug resistance outer membrane protein MdtQ | |
| 81 | NP_756598.1 | c4739 | 0.43 | 0 | − | − | Hypothetical protein c4739 | |
| 82 | NP_753585.1 | ychP | 0.435 | 0 | − | − | Hypothetical protein c1680 | |
| 83 | NP_754913.1 | c3031 | 0.448 | 0 | − | − | SinH-like protein | |
| 84 | NP_752491.1 | ybaU | 0.464 | 1 | − | − | Peptidyl-prolyl cis-trans isomerase (rotamase D) | |
| 85 | NP_755264.1 | c3389 | 0.282 | 3 | X | − | − | Hypothetical protein |
Note: The protein RefSeq numbers are from UPEC strain CFT073. All 85 proteins are conserved across total four UPEC genomes. This table also shows the adhesin probability, number of transmembrane helixes, and absence in nonpathogenic K-12 MG1655. TMH, transmembrane alpha helix prediction. Microarray, transcriptomic mRNA results (+ for up-regulation in vivo). Proteomics, protein expression results (+ for up-regulation in urine or in vivo).