Figure 1.

TNF-α and IL-12 production and NF-κB activation in response to M. leprae infection in macrophages from A/J and C57BL/6 mice. Macrophages (10⁶) from C57BL/6 and A/J mice were treated with LPS (100 ng/ml) and M. leprae (MOI of 0.1, 1.0, and 10.0) for 18 h, and supernatants and cell extracts were assayed for cytokines (IL-12 and TNF-α) and NF-κB, respectively. Open bar and closed bar represent macrophages from C57BL/6 and A/J mice, respectively. Data are representative of at least three independent experiments, each performed in triplicate.