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. Author manuscript; available in PMC: 2010 Jul 27.
Published in final edited form as: Neuropharmacology. 2008 Nov 7;56(2):541–555. doi: 10.1016/j.neuropharm.2008.10.012

Figure 7. Protein and intranuclear mRNA expression of the KCC2 co-transporter at three developmental stages in layer II/III of the neocortex.

Figure 7

(A1 – A3) 40× confocal images of immunohistochemical experiments performed with an anti-KCC2 antibody (red) and the nuclear stain, DAPI (blue) (Scale bar 10 μm). (A4–A6) 40× confocal images of FISH assays that were performed with a KCC2 specific mRNA riboprobe (red) and the nuclear stain, DAPI (blue) (B) KCC2 protein fluorescence intensity (arbitrary units) divided by the number of cell per field as a function of developmental stage (n= 6 animals per age group, ** p< 0.01 by repeated measures one-way ANOVA followed by Tukey’s post-hoc test). (C) Percent of cells that were positive for KCC2 mRNA foci per field as a function of developmental stage (n= 6 animals per age group, *** p< 0.001 by repeated measures one-way ANOVA followed by Tukey’s post-hoc test.