Figure 1.
Acute exposure of HUVECs to Ang II selectively decreases GTPCH I activity and reduces levels of total biopterins, BH4, and GTPCH I. A, Levels of total biopterins and BH4 after a 6-hour exposure to Ang II (0.1 μmol/L). Cells were also analyzed for changes in (B) GTPCH I protein levels and (C) total GTPCH I activity. The blot is representative of 3 independent experiments. Results (n=3) were analyzed with a 1-way ANOVA.