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. Author manuscript; available in PMC: 2010 Jul 27.
Published in final edited form as: Hypertension. 2009 Jul 13;54(3):625–632. doi: 10.1161/HYPERTENSIONAHA.109.133736

Figure 6.

Figure 6

Ang II–induced hypertension enhances 26S proteasome activation, which decreases aortic levels of GTPCH I and BH4 and impairs endothelium-dependent vessel relaxation. Mice were infused with Ang II (0.85 mg/kg per day for 14 days) or saline and also injected with MG132 (IP, 5 mg/kg of body weight for 2 days) or vehicle on the last 2 days of Ang II or saline infusion (n=6 for all 4 groups). Aortas were then collected for analysis of (A) GTPCH I protein levels, (B) total biopterins and BH4 levels, (C) tyrosine-nitrated PA700, (D) 26S proteasome activity, and (E) endothelium-dependent relaxation. F, Blood pressures were measured in conscious animals to compare the differences in the increase of blood pressure by Ang II infusion without MG132 treatment and those with MG132 treatment. Results (n=6) were analyzed with ANOVA. *P<0.05 vs vehicle, #P<0.05 vs Ang II alone. n.s. indicates not significant.