Table 1.
Participant Characteristics for Each Part of the Study
| Cases | Controls | |
|---|---|---|
| Gene Array Samples | ||
| Age (range), y | 67 (49–80) | 65 (44–80) |
| Sex | ||
| Male | 3 | 3 |
| Female | 8 | 8 |
| Total RNA yield (ng/μL) | 18.72 (8.4–63) | 15.8 (10.2–64.4) |
| CT/NG Amplicor | NT | NT |
| Median number (range) of genes expressed per array type | ||
| ECM | 40 (29–64) | 49 (36–65) |
| Th1/Th2/Th3 | 33 (19–55) | 48 (21–63) |
| qRT-PCR Samples | ||
| Age (range), y | 54 (3–76) | 50 (3–75) |
| Sex | ||
| Male | 22 | 22 |
| Female | 25 | 25 |
| Total RNA yield (ng/μL) | 13.75 (6.4–29.2) | 13.64 (2.9–22.8) |
| Total DNA* (qPCR) | 3300 (67–13790) | 3536 (27–39840) |
| Total protein (ng/μL) | 309.8 (100–794) | 331.4 (119–791) |
| CT/NG Amplicor (n positive) | 1 | 0 |
n = 11 for both cases and controls for Gene Array; n = 47 for both groups for qRT-PCR. Data are expressed as median (range). NT, not tested.
*
Below measurable range (<2 ng/μL) of the spectrometer (OD 280 nm) and therefore estimated by relative quantification real-time PCR.