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Karyotype analyses of primary (passage 2) human meibomian gland epithelial cells, as well as cells immortalized with hTERT or SV40 large T and hTERT. The 46, XY karyotype of the hTERT-immortalized human meibomian gland epithelial cells at passage 23 was normal and identical with that of the male parent primary cells. The karyotype of human meibomian gland epithelial cells immortalized with both SV40 large T antigen and hTERT was abnormal.
