Table 1.

Examples of Lipid-, Keratin- and Protease-Related Genes Expressed in the Various Tissues Studied

Gene ID	Gene	Immortalized SGE Cells	hTERT-Immortalized MGE Cells	Primary Human MGE Cells	Human Meibomian Gland
ACADM	Acyl-coenzyme A dehydrogenase	100	100	100	100
FDPS	Farnesyl diphosphate synthase	100	100	100	100
FDFT1	Farnesyl-diphosphate farnesyltransferase 1	100	100	100	100
FNTA	Farnesyltransferase, CAAX box, α	100	100	100	100
FNTB	Farnesyltransferase, CAAX box, β	100	100	100	100
FADS1	Fatty acid desaturase 1	100	100	75	100
FASN	Fatty acid synthase	100	100	100	100
KLK6	Kallikrein-related peptidase 6, Variant A	100	93	100	100
KLK6	Kallikrein-related peptidase 6, Variant B	100	100	100	92
KRT7	Keratin 7	100	100	100	100
KRT13	Keratin 13	100	100	100	100
SQLE	Squalene epoxidase	100	100	100	100
SCD	Stearoyl-CoA desaturase	100	100	100	100
SREBF1	Sterol regulatory element binding transcription factor 1	100	100	100	100

Data are the percentage of samples of immortalized human sebaceous gland epithelial (SGE) cells (n = 6) primary (n = 12) and hTERT-immortalized human meibomian gland epithelial (MGE) cells (n = 30), and human meibomian glands (n = 12) that contained the designated genes. The data for immortalized sebocytes are reported in Gene Expression Omnibus Series accession number GSE10432 (NCBI, Bethesda, MD, http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE10432).²⁷ Analogous gene expression findings are published for ACADM, FNTA, FNTB, FADS1, FASN, KRT7, KRT13, and SCD in immortalized sebocytes and/or sebaceous glands.^26,28,38 The primary and immortalized meibomian gland epithelial cells were cultured under a variety of conditions, including exposure to exogenous calcium, FBS, EGF, BPE, or DHT (i.e., Liu S, et al. IOVS:2008;49:ARVO E- Abstract 88; Liu S, et al. IOVS 2009;40:ARVO E-abstract 3669; Khandelwal P, et al. IOVS 2009;50:ARVO E-abstract 4266; Khandelwal P, et al. IOVS 2010;51:ARVO E-abstract 4158).