Table 10.

Comparison of Our Study with Previous Microarray Studies Using Whole Retina Glaucoma Model

	Our Study	Miyahara et al.7	Ahmed et al.8	Piri et al.9	Steele et al.6	Naskar et al.10	Yang et al.11		Walsh et al.12
Species	Brown Norway rat	Cynomolgus monkey	Brown Norway rat	Wistar rat	DBA2J mouse	Rat	Wistar rat		C57 Mice
Glaucoma model	Saline injection	Laser-induced glaucoma	Saline injection	Optic nerve transsection	8 months after IOP elevation	Hereditary high IOP	Laser-induced glaucoma	Optic nerve transsection	Saline injection
Samples	RGC	Whole retina	Whole retina	Whole retina	Whole retina	Whole retina	Whole retina		Whole retina
Array*	Affymetrix rat 230.2	UniGEM Human ver 2.0	Affymetrix rat U34A	Aligent rat G4130A	Affymetrix mouse 430.2	MWG Biotech 10K chip	Affymetrix rat 230.2		Affymetrix mouse 430.2
Statistic setting	>2-fold change	>1.7-fold change	>2-fold change	>2-fold change	>1.8-fold change	>3-fold change	>2-fold change		>1.5-fold change
Number of upregulated genes	330	45	74	85	32	75	347	212	50
Number of downregulated genes	575	17	7	21	36	45	196	480	140
Major upregulated genes	Apoptosis genes (Table 4), Complement component genes (C1qa, C1qb, C3)	Immune response genes, glia activation genes, ceruloplasmin	Immune response genes, glia activation genes	Apoptotic genes, ceruloplasmin	Glial activation, immune response genes, signaling, gene expression	Glial activation genes	Early phase: immune and stress response genes. Late phase: genes related to protein synthesis	Early phase: genes related to cell death	Saa1, Saa2
Major downregulated genes	Pro-survival genes (Cpg15, Nrg1, Ywhaz, Ywhab, Grb2, Nfl, Stmn2, Map2k1, Nell2, Vsnl1), neurogenesis genes (Gja1, Snap25, Cit, Nrep, Gabrg2 and Vdac3)	Neurofilament genes	Neurofilament genes	Neurofilament genes, Neuritin (Cpg15)	Multiple crystal genes			RGC-enriched genes; neurofilament genes

^*Affymetrix, Santa Clara, CA; Agilent, Palo Alto, CA; UniGem, Incyte Genomics, Palo Alto, CA; MWG Biotech, High Point, NC.