Table 10.
Our Study | Miyahara et al.7 | Ahmed et al.8 | Piri et al.9 | Steele et al.6 | Naskar et al.10 | Yang et al.11 | Walsh et al.12 | ||
---|---|---|---|---|---|---|---|---|---|
Species | Brown Norway rat | Cynomolgus monkey | Brown Norway rat | Wistar rat | DBA2J mouse | Rat | Wistar rat | C57 Mice | |
Glaucoma model | Saline injection | Laser-induced glaucoma | Saline injection | Optic nerve transsection | 8 months after IOP elevation | Hereditary high IOP | Laser-induced glaucoma | Optic nerve transsection | Saline injection |
Samples | RGC | Whole retina | Whole retina | Whole retina | Whole retina | Whole retina | Whole retina | Whole retina | |
Array* | Affymetrix rat 230.2 | UniGEM Human ver 2.0 | Affymetrix rat U34A | Aligent rat G4130A | Affymetrix mouse 430.2 | MWG Biotech 10K chip | Affymetrix rat 230.2 | Affymetrix mouse 430.2 | |
Statistic setting | >2-fold change | >1.7-fold change | >2-fold change | >2-fold change | >1.8-fold change | >3-fold change | >2-fold change | >1.5-fold change | |
Number of upregulated genes | 330 | 45 | 74 | 85 | 32 | 75 | 347 | 212 | 50 |
Number of downregulated genes | 575 | 17 | 7 | 21 | 36 | 45 | 196 | 480 | 140 |
Major upregulated genes | Apoptosis genes (Table 4), Complement component genes (C1qa, C1qb, C3) | Immune response genes, glia activation genes, ceruloplasmin | Immune response genes, glia activation genes | Apoptotic genes, ceruloplasmin | Glial activation, immune response genes, signaling, gene expression | Glial activation genes | Early phase: immune and stress response genes. Late phase: genes related to protein synthesis | Early phase: genes related to cell death | Saa1, Saa2 |
Major downregulated genes | Pro-survival genes (Cpg15, Nrg1, Ywhaz, Ywhab, Grb2, Nfl, Stmn2, Map2k1, Nell2, Vsnl1), neurogenesis genes (Gja1, Snap25, Cit, Nrep, Gabrg2 and Vdac3) | Neurofilament genes | Neurofilament genes | Neurofilament genes, Neuritin (Cpg15) | Multiple crystal genes | RGC-enriched genes; neurofilament genes |
Affymetrix, Santa Clara, CA; Agilent, Palo Alto, CA; UniGem, Incyte Genomics, Palo Alto, CA; MWG Biotech, High Point, NC.