. 2010 Aug;51(8):4084–4095. doi: 10.1167/iovs.09-4864

Table 9.

Validation of Microarray Gene Expression by Real-Time Quantitative PCR

Gene	Ratio of Change (Mean ± SD)
Gene	Microarray (n = 3)	RT-qPCR (n = 5)
Ywhaz: 14–3-3 family, zeta polypeptide	−5.03 ± 0.89^*	−25.77 ± 5.42^*
Ywhab: 14–3-3 family, beta polypeptide	−3.33 ± 0.55^*	−23.14 ± 6.26^*
Cpg15: candidate plasticity gene 15	−2.61 ± 0.30^*	−20.93 ± 9.79^*
Grb2: growth factor receptor bound protein 2	−2.34 ± 0.32^*	−24.59 ± 10.43^*
Nrg1: neuregulin 1	−2.06 ± 0.30^*	−6.56 ± 0.47^*
Map2k1: mitogen activated protein kinase kinase 1	−2.06 ± 0.23^*	−9.73 ± 3.43^*
C3: complement component 3	3.66 ± 1.01^*	5.07 ± 1.55^*
C1qa: complement component 1, q subcomponent, alpha polypeptide	1.39 ± 0.32	1.89 ± 0.45
Stat3: signal transducer and activator of transcription 3	3.06 ± 0.53^*	7.28 ± 1.27^*
Stat5a: signal transducer and activator of transcription 5a	2.26 ± 0.55^*	2.74 ± 0.90^*
C1qb: complement component 1, q subcomponent, beta polypeptide	1.28 ± 0.11	2.13 ± 0.56^*

P < 0.05.