Table 1.
Chronobiological data derived from best fitting sine curves (fitted period:24 hours = 360°) and F and p value from ANOVA performed between the timepoints for each variable
| Healthy subjects | Lung cancer patients | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Factor | p# | MESOR ± SE | Amplitude ± SE | Acrophase ± SE | ANOVA | p# | MESOR ± SE | Amplitude ± SE | Acrophase ± SE | ANOVA | ||||||
| Original Units | % of mean | Original Units | % of mean | |||||||||||||
| F | p | F | p | F | p | F | p | |||||||||
| Cortisol | 0.027 | 12.52 ± 1.53 | 5.85 ± 1.97 | 08:08 ± 01:18 | 6.3 | 0.01 | 22.3 | <0.01 | 0.138 | 17.85 ± 1.03* | 4.21 ± 1.39 | 09:00 ± 01:32 | 1.1 | 0.41 | 5.1 | <0.01 |
| Melatonin | 0.013 | 38.75 ± 5.43 | 26.24 ± 7.32 | 01:13 ± 01:15 | 14.2 | <0.01 | 25.4 | <0.01 | 0.036 | 33.12 ± 7.34 | 23.12 ± 9.94 | 01:28 ± 01:37 | 4.9 | <0.01 | 12.3 | <0.01 |
| TRH | 0.024 | 0.45 ± 0.09 | 0.04 ± 0.05 | 02:47 ± 01:05 | 0.1 | 0.97 | 4.5 | 0.01 | 0.245 | 0.60 ± 0.02* | 0.03 ± 0.04 | 23:41 ± 01:48 | 0.3 | 0.35 | 0.5 | 0.46 |
| TSH | 0.020 | 1.38 ± 0.04 | 0.45 ± 0.03 | 23:12 ± 00:32 | 2.3 | 0.08 | 12.5 | 0.01 | 0.328 | 1.33 ± 0.09* | 0.18 ± 0.08 | 00:05 ± 02:05 | 0.6 | 0.78 | 3.9 | 0.01 |
| FT4 | 0.897 | 1.24 ± 0.06 | 0.01 ± 0.03 | 15:52 ± 08:08 | 0.3 | 0.95 | 1.4 | 0.15 | 0.377 | 1.33 ± 0.02 | 0.02 ± 0.03 | 18:44 ± 02:13 | 0.4 | 0.96 | 0.8 | 0.72 |
| GH | 0.039 | 0.38 ± 0.03 | 0.45 ± 0.02 | 00:22 ± 00:50 | 3.9 | 0.01 | 5.8 | <0.01 | 0.447 | 1.55 ± 0.17* | 0.38 ± 0.23 | 22:16 ± 02:36 | 0.5 | 0.61 | 1.3 | 0.23 |
| IGF-1 | 0.578 | 221.01 ± 6.0 | 9.34 ± 8.12 | 08:28 ± 03:24 | 0.2 | 0.76 | 3.4 | 0.09 | 0.424 | 156.02 ± 1.88* | 4.05 ± 2.36 | 02:41 ± 02:26 | 0.1 | 0.89 | 1.7 | 0.29 |
| CD3 | 0.014 | 82.10 ± 0.31 | 0.98 ± 0.53 | 03:17 ± 22:09 | 0.3 | 0.96 | 2.3 | 0.04 | 0.135 | 83.01 ± 0.21 | 1.08 ± 0.25 | 20:49 ± 01:21 | 0.7 | 0.76 | 1.9 | 0.21 |
| CD4 | 0.033 | 45.12 ± 0.75 | 3.11 ± 1.02 | 01:16 ± 01:17 | 1.5 | 0.36 | 4.0 | <0.01 | 0.035 | 43.83 ± 1.27 | 6.13 ± 1.47 | 22:48 ± 00:53 | 2.3 | 0.04 | 6.2 | 0.01 |
| CD8 | 0.024 | 29.61 ± 0.74 | 2.59 ± 1.04 | 12:48 ± 01:37 | 0.4 | 0.76 | 4.2 | 0.01 | 0.531 | 23.12 ± 0.36* | 0.52 ± 0.46 | 11:21 ± 03:04 | 0.4 | 0.98 | 0.9 | 0.61 |
| CD8 Bright | 0.039 | 21.06 ± 0.54 | 1.35 ± 072 | 13:34 ± 02:04 | 0.6 | 0.84 | 1.4 | 0.04 | 0.597 | 15.21 ± 0.26* | 0.42 ± 0.36 | 04:45 ± 03:19 | 0.3 | 0.93 | 0.7 | 0.72 |
| CD8 Dim | 0.028 | 9.22 ± 0.39 | 1.10 ± 0.49 | 12:37 ± 01:58 | 1.5 | 0.35 | 3.7 | 0.01 | 0.631 | 9.45 ± 0.43 | 0.82 ± 0.69 | 12:46 ± 03:40 | 0.6 | 0.62 | 1.2 | 0.39 |
| CD16 | 0.015 | 7.89 ± 0.21 | 1.42 ± 0.36 | 13:24 ± 01:37 | 0.7 | 0.51 | 2.8 | 0.01 | 0.187 | 16.33 ± 0.41* | 2.12 ± 0.37 | 11:16 ± 01:32 | 0.5 | 0.81 | 2.3 | 0.05 |
| CD20 | 0.006 | 11.21 ± 0.04 | 1.21 ± 0.08 | 21:24 ± 00:21 | 0.4 | 0.98 | 2.3 | 0.09 | 0.129 | 8.73 ± 0.12 | 0.61 ± 0.45 | 02:21 ± 01:16 | 0.3 | 0.89 | 1.0 | 0.56 |
| CD25 | 0.040 | 5.52 ± 0.12 | 0.44 ± 0.19 | 19:46 ± 01:34 | 0.2 | 0.93 | 2.4 | 0.08 | 0.526 | 7.02 ± 0.31 | 0.68 ± 0.32 | 20:12 ± 03:04 | 0.6 | 0.80 | 3.9 | <0.01 |
| HLA-DR | 0.033 | 15.28 ± 0.39 | 0.69 ± 0.56 | 22:33 ± 03:21 | 0.6 | 0.67 | 2.2 | 0.07 | 0.893 | 14.02 ± 0.41 | 0.28 ± 0.49 | 18:47 ± 08:06 | 0.1 | 0.54 | 0.5 | 0.83 |
| TcR δ 1 | 0.026 | 3.59 ± 0.11 | 0.41 ± 0.18 | 11:12 ± 01:33 | 0.2 | 0.89 | 3.2 | 0.04 | 0.082 | 2.24 ± 0.02* | 0.94 ± 0.15 | 14:09 ± 00:22 | 1.2 | 0.39 | 3.6 | 0.01 |
| δTcS1 | 0.036 | 1.43 ± 0.05 | 0.43 ± 0.06 | 10:52 ± 00:44 | 0.4 | 0.76 | 2.9 | 0.03 | 0.918 | 0.91 ± 0.20* | 0.07 ± 0.18 | 14:56 ± 09:32 | 0.2 | 0.78 | 2.1. | 0.08 |
| IL-2 | 0.424 | 0.43 ± 0.02 | 0.05 ± 0.03 | 14:53 ± 02:32 | 0.2 | 0.98 | 0.3 | 0.94 | 0.627 | 0.52 ± 0.03* | 0.02 ± 0.04 | 17:01 ± 03:10 | 0.4 | 0.86 | 1.0 | 0.05 |
Units: μg/dl for cortisol, pg/ml for melatonin, ng/ml for TRH, μU/ml for TSH, ng/dl for FT4, ng/ml for GH, ng/ml for IGF-I, percentage for lymphocyte subpopulations, IU/ml for IL-2; all parameters analyzed in all the subjects; Hr:Mn for acrophase; all parameters analyzed in all the subjects. # p value from an F-test of the null amplitude rejection hypothesis (for a rhythm with a chosen period τ); * p value < 0.05 from t-test or Mann-Whitney rank sum test as indicated performed on MESOR, amplitude and acrophase values