Table 3.

Results of the mutation analyses of KIT exon 11 performed in trial 3.

	Lab A						Lab C						Lab D						Lab E						Lab F
Sample	T3.1		T3.2		T3.3		T3.1		T3.2		T3.3		T3.1		T3.2		T3.3		T3.1		T3.2		T3.3		T3.1		T3.2		T3.3
Experimental Step	A	S	A	S	A	S	A	S	A	S	A	S	A	S	A	S	A	S	A	S	A	S	A	S	A	S	A	S	A	S
Primer Lab A/B	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+/-	+	+	+	+	+	+
Primer Lab C/D	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+/-	+	+	+	+	+	+
Primer Lab E	+/-	-	+/-	-	+/-	-	-	-	-	-	-	-	+	+	+	-	+	+/-	+	+	+	+	+	+/-	+	+/-	+	+/-	+	+/-
Primer Lab F	+/-	-	+	+	+	+/-	+	+	+	+	+	+	+	-	+	+	+	-	+	-	+	+	+	-	+	-	+	+	+	+

For each lab the result of amplification (A) and sequence analysis (S) is detailed. "+" means success of amplification or nominal result in sequence analysis, "+/-" means only faint amplification with unspecific extra-bands or different/not-scorable sequence-analysis, and "-" means failure of amplification or sequence analysis. Primer pairs used in Lab A and B and Lab C and D were the same, respectively. Lab B did not participate in the third trial suppl.