Table 2.

Fitting parameters for CaM 34-110 in high and low Ca²⁺ buffers. All fits contained a static FRET component; f_T is the triplet fraction; τ_T is the triplet time constant; τ_D is the transit time.

Fit Parameter	High Ca2+c	Low Ca2+c
〈N〉	3.13 ± 0.02	2.37 ± 0.02
fT (green)	0.1	0.1
τT (green) (μs)	8	8
fT (red)	0.09	0.09
τT (red) (μs)	18	18
τD (μs)a	281 ± 6	264 ± 6
τD RR (μs)b	246 ± 5	247 ± 8
τ1 (μs)	${92}_{-60}^{+170}$	n.a.
χ2	1.42	2.53

^aTransit time for G_GG(τ), G_GR(τ), and G_RG(τ).

^bThe transit time for G_RR(τ) was allowed to vary independently to account for photobleaching of the acceptor during transit through the focal volume.

^cUncertainties were calculated by the support plane method with confidence range 1 σ.