Figure 8. B5 silencing has more modest effects on HIV or VSV infection.

(A) Jurkat-TAT-CCR5 cells were transfected with siHVEM (control) or B5 specific siRNA and 48 h post-transfection, immunoblots of cell lysates were prepared and probed with anti-B5 polyclonal Ab (upper panel). Transfected cells were infected with HSV-1(KOS) and 4 h pi, cell lysates prepared and analyzed for ICP0 expression (middle panel) and β-actin as a loading control (lower panel). In parallel, the transfected cells were infected with HIV-1(BaL) and culture supernatants harvested 5 days pi and analyzed for p24 expression (right). (B) CaSki cells were transfected with HVEM or B5 specific siRNA and 48 h post-transfection immunoblots of cell lysates were prepared and probed with anti-B5 polyclonal Ab (upper) and β-actin as a loading control (lower panel). The transfected cells were infected with HSV-2(G) or VSV and plaques counted 48 h pi; results are means ± sd obtained from two independent experiments conducted in duplicate.