Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Tracking of NF- κB translocation from the cytosol to the nucleus over time. The intracellular proteins were extracted by flow-through electroporation and the supernatant and pellet fractions were analyzed by Western blotting after stimulation of CHO/GFP-NFκBp65 cells by IL-1β for different periods of time. We used 800 V/cm and 50 ms for the electroporation.
