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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Thromb Res. 2010 Aug;126(2):130–136. doi: 10.1016/j.thromres.2010.04.010

Fig 1. Generation of HepG2 cells that constitutively express allelic F.II reporter genes.

Fig 1

Chimeric TRE-βF.IIG and -βF.IIA DNAs comprise elements of the human prothrombin and β-globin genes (black and gray, respectively) linked to a TRE promoter (crosshatched). The two genes are identical except for a G→A substitution at position 20210. (B) Isolation of HepG2 subclones that express βF.IIG and βF.IIA mRNAs. Total RNA from representative hygromycin-resistant clones expressing βF.IIG and βF.IIA mRNAs was analyzed by RNase protection using [32P]-labeled β-globin and control β-actin RNA probes. Representative autoradiographs are illustrated. PC = positive control β-globin mRNA.