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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Am J Obstet Gynecol. 2010 Jun 11;203(2):185.e5–185.e11. doi: 10.1016/j.ajog.2010.05.003

Figure 2.

Figure 2

Hyperglycemia increases iNOS transcription and induces nitrosative stress. PYS-2 cells were transfected with iNOS-luc and cultured under euglycemic and hyperglycemic conditions in the presence or absence of 400 nM SP600125. In A, high glucose dose-dependently increased iNOS-luc activities. In B, iNOS-luc activities were significantly increased in hyperglycemia group (Hyper: 450 mg/dl glucose) group than that in the euglycemia group (90 mg/dl glucose). SP600125 blocked hyperglycemia-induced iNOS-luc activities. C showed representative images of DAF-FM diacetate immunofluorescence. Bar represented 0.1 mm. Hyper: hyperglycemia (450 mg/dl glucose). In D, representative images of nitrosylated proteins were shown. The arrow pointed the most elevated band of nitrosylated protein by hyperglycemia. Experiments were repeated three times and same results were obtained. Data expressed as Mean ± SE (n = 3). * denotes significant difference (p<0.05) compared to other groups when analyzed by one-way ANOVA and Tukey test.