Figure 1. SNaPshot analysis of rs2229992 SNP as a quantitative test.
Regression analysis of the amplification products of two homozygous control cDNAs mixed in different proportions and analyzed in triplicate by SNaPshot. Allele frequencies were calculated by the peak heights: freq. C = C/(C+kT), where the correction factor k is determined from the mix simulating an actual allele frequency of 0.5 (5:5). Measured allele frequencies were plotted against the expected values. A near-linear relationship was observed across all data points (R2 = 0.9983).