Table 1.
Effect of the β-NRX SS4 insert and NL A/B inserts on the β-NRX/NL binding KDs
| KD(μM) | Fold-change compared to | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| β-NRX 1Δ4 | β-NRX 2Δ4 | β-NRX 3Δ4 | β-NRX 1+4 | β-NRX 2+4 | β-NRX 3+4 | β-NRX 1Δ4/NLΔ | β-NRX 2Δ4/NLΔ | β-NRX 3Δ4/NLΔ | β-NRX 1+4/NLΔ | β-NRX 2+4/NLΔ | β-NRX 3+4/NLΔ | |
| NL1Δ | 0.828(2) | 0.917(5) | 1.02(1) | 1.54 (1) | 3.77(3) | 0.3320(1) | 1.00 | 1.00 | 1.00 | 1.86 | 4.11 | 0.33 |
| NL2Δ | 0.964(2) | 0.7055(8) | 0.418(2) | 3.520(9) | 3.388(7) | 0.262(5) | 1.00 | 1.00 | 1.00 | 3.65 | 4.80 | 0.63 |
| NL3Δ | 8.17(3) | 16.48(3) | 10.1(3) | 23.8(3) | 56.8(3) | 9.73(1) | 1.00 | 1.00 | 1.00 | 2.91 | 3.45 | 0.96 |
| NL1A | 0.845(3) | 1.466(7) | 0.782(3) | 2.096(4) | 3.317(7) | 0.2460(4) | 1.02 | 1.60 | 0.77 | 2.53 | 3.62 | 0.24 |
| NL1B | 1.16 (1) | 2.21(1) | 1.10(8) | 2.754(8) | 5.48(2) | 0.3650(8) | 1.40 | 2.41 | 1.08 | 3.33 | 5.98 | 0.36 |
| NL1AB | 1.21 (6) | 2.29 (5) | 1.10(7) | 2.82(1) | 5.66(2) | 0.393(1) | 1.46 | 2.50 | 1.08 | 3.41 | 6.17 | 0.39 |
| NL2A | 0.430(5) | 0.65 (1) | 0.369(8) | 3.168(9) | 3.013(8) | 0.239 (3) | 0.45 | 0.92 | 0.88 | 3.29 | 4.27 | 0.57 |
| NL3A1 | 7.45(3) | 9.05(8) | 6.26(3) | 12.0(5) | 20.5(1) | 4.32(1) | 0.91 | 0.55 | 0.62 | 1.47 | 1.24 | 0.43 |
| NL3A2 | 7.15(5) | 23(3) | 11.2(1) | 21.9(1) | 42.2(8) | 9.50(2) | 0.88 | 1.40 | 1.11 | 2.68 | 2.56 | 0.94 |
| NL3A1A2 | 6.57(3) | 15.34(9) | 10.9(8) | 21.0(1) | 48(1) | 9.39(1) | 0.80 | 0.93 | 1.08 | 2.57 | 2.91 | 0.93 |
The left side of the table shows KD values for the interactions of isoforms of NLs 1, 2 and 3 with isoforms of β-NRXs 1, 2 and 3. The blue quadrant shows the KDs for interactions where both NLs and β-NRXs lack a spice insert (A or B for NLs and SS4 for β-NRXs). The green quadrant displays the KDs for NLs that contain inserts A or B with β-NRXs that lack splice insert 4. The orange quadrant describes the binding of NLs that lack a splice insert with β-NRXs containing insert 4, and the purple quadrant shows the KDs for the interaction of both insert-containing NLs and β-NRXs. The right side of the table shows all the KD values normalized against the same NL/β-NRX pair that lacks both inserts, which are in the left blue quadrant. The number in brackets represents the error of the fit in the last digit.