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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: J Immunol. 2010 Apr 21;184(11):6076–6083. doi: 10.4049/jimmunol.0902300

FIGURE 6.

FIGURE 6

Th1 cells or IFN-γ are necessary for the rejection of corneal allografts in mice with AC. Th1 (Tim3+) and Th2 (T1/ST2+) cells were isolated from the spleens of SRW pollen-sensitized mice that had rejected C57BL/6 corneal allografts. A, One donor-equivalent of Th2 cells or a combination of Th1 + Th2 cells was adoptively transferred to BALB/c nude mice. The effect of recombinant murine IFN-γ as a substitute for Th1 cells was evaluated by injecting one donor-equivalent of Th2 cells, followed by i.p. injection of recombinant murine IFN-γ (1.25 µg) on the day of transplantation and twice per week thereafter. B, One donor-equivalent of Th1 cells was adoptively transferred to BALB/c nude mice. One panel of mice received Th1 cells, followed by i.p. injection of recombinant murine IFN-γ (1.25 µg) on the day of transplantation and twice per week thereafter. This experiment was performed twice with similar results (n = 7–10 mice per group). p = 0.003 (Th1 alone versus Th1 + Th2); p = 0.003 (Th2 versus Th1 + Th2); p > 0.05 (Th2 + Th1 versus Th2 + IFN-γ); p > 0.05 (Th1 + IFN-γ versus Th1 alone).