Skip to main content
. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Ann Otol Rhinol Laryngol. 2010 Apr;119(4):270–278. doi: 10.1177/000348941011900411

Fig 2.

Fig 2

Images of agarose gels (1.5%) stained with EtBr. Expression of cysteine leukotriene (CysLT1R) and von Willebrand factor (vWF) genes in A) normal chinchilla middle ear mucosa (MEM) and B) Haemophilus influenzae–infected MEM. –T — no template RNA/DNA added to polymerase chain reaction (PCR); –RT — PCR performed without prior reverse transcriptase step in presence of template RNA; +T — template RNA plus reverse transcription (RT) before PCR. +T reaction using CysLT1R-specific primers from both normal and infected MEM yield predicted 211 bp PCR product, and +T reactions for vWF gene–specific primers yielded expected 266 bp PCR product.