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. Author manuscript; available in PMC: 2010 Jul 28.
Published in final edited form as: Pathogenesis (Amst). 1999;1(3):201–211.

FIGURE 3.

FIGURE 3

Analysis of C. pneumoniae from AD brain homogenates. Panel A: THP-1 cells after infection with a homogenate from the temporal cortex from an AD brain 7 d post-incubation. Immunohistochemical analysis with an anti-OMP mAb shows organisms (arrows) within the cytoplasm of the cells. Bar=20 μm. Panel B: Ultrastructural analysis of infected THP-1 cells with C. pneumoniae from an AD brain homogenate 7 d post-incubation. Organisms demonstrating a more aberrant morphology can be seen in the cytoplasm of the host cell (arrow). The EBs and RBs show thickened cell walls and are variable in size. Since the bacteria are dependent on the host-cell's ATP, C. pneumoniae bodies are situated adjacent to mitochondria (arrowhead) which shows morphology indicative of mitochondria undergoing oxidative stress (Parker et al., 1990). Bar=1 μm. Panel C: Cloned 350 bp PCR product from the infected THP-1 cells shown in the previous two panels. (Left) Lane 1:100 bp standards. Lane 2: Uncut ligated M13 vector. Lane 3: Restriction digest of vector in lane 2; the lower band was sequenced and found to be Homologous to the C. pneumoniae omp A gene