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. Author manuscript; available in PMC: 2010 Jul 28.
Published in final edited form as: J Stem Cells. 2007;2(3):139–147.

Figure 3.

Figure 3

Human embryonic stem cells express osteoblastic markers when co-cultured with differentiated hBMSCs. hBMSCs were grown on transwell inserts in osteogenic induction medium for 7, 14 or 24 days. Differentiated hBMSCs were co-cultured with hESCs for an additional 28 days. Lane 1: undifferentiated hESCs; lane 2: hESC in growth medium; lane 3: hESC in osteogenic medium; lane 4: transwell co-culture with undifferentiated hBMSCs (control); lane 5: transwell co-culture with 7 day differentiated hBMSCs; lane 6: transwell co-culture with 14 day differentiated hBMSCs; lane 7: transwell co-culture with 24 day differentiated hBMSCs.