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Human embryonic stem cells express osteocalcin when co-cultured with differentiated hBMSCs. hBMSCs were grown on transwell inserts in osteogenic induction medium for 24 days. Differentiated hBMSCs were co-cultured with hESCs for an additional 28 days. Expression of osteocalcin with DAPI counterstaining of hESC co-cultures with undifferentiated hBMSCs (A-C) and 24 day differentiated hBMSCs (D-F). Magnification = 10×.
