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. 2009 May-Aug;15(2):65–71. doi: 10.4103/0971-6866.55218

Figure 1.

Figure 1

Restriction fragment length polymorphism analysis of Hsp70-2 gene: The Hsp70-2 PCR products(189bp) were digested with Pst1. The Hsp70-2A/Aallele corresponds to 116 and 73bp cleavage products (presence of Pst1 site) and hsp70-2G/G allele corresponds to 189bp uncleaved product (absence of Pst1 site). The reaction products electrophoresed on 3% agarose gel are shown. Lane#1: 50bp Molecular Weight Marker; Lane# 2, 4, 6: undigested HSP 70.2 189bp PCR product; Lane # 3, 5, 7 = Restriction enzyme Pst1 digested HSP 70.2 PCR product (all showing heterozygous change, hsp70-2A/G)