Figure 3.
ROL5 Is Homologous to Ncs6p of Yeast.
The alignment of ROL5 with Ncs6p of S. cerevisiae reveals 54% identity and 70% similarity between the two proteins. The Ncs6p-like proteins of different organisms share common motifs that are indicated below the sequences [(CxxC)2 – SGGxDS – CxxC – GH – PL – C – (CxxC)2], all of which are conserved between the two proteins. The motif PL is not fully conserved in Ncs6p and ROL5. Sequences important for protein activity (Björk et al., 2007) are boxed. The Gly-65 to Asp mutation in rol5-1 (star) is adjacent to the PP-loop motif SGGxDS, which is important for ATP binding.