Figure 4.

Ncs6p and ROL5 Have Similar Functions.

(A) tRNA was extracted from 7-d-old wild-type and rol5-1 seedlings and separated on an acrylamide gel with (left panel) or without (right panel) APM, a compound that interacts with the 2-thiouridine and retards migration in the gel.

(B) Wild-type (WT) and Δncs6 mutant yeast was grown in the absence or presence of rapamycin. The Δncs6 mutant grew normally on control medium but was hypersensitive to rapamycin compared with the wild type. Expression of ROL5 but not rol5-1 under the control of the yeast PHOSPHOGLYCERATE KINASE promoter in the Δncs6 mutant suppressed this rapamycin hypersensitivity phenotype. Spots on a line represent serial dilutions (10-fold).