Figure 6.

Immunolabeling of Cell Walls of rol5-1 and Rapamycin-Treated Wild-Type Seedlings.

Immunolabeling of 4-d-old roots with monoclonal antibodies (1→4)-β-d-galactan side chains of RG I (LM5) and glucuronic acid–containing side chains of arabinogalactan proteins (LM2).

(A) Compared with the wild type (WT), the rol5-1 mutant root surface revealed reduced detection of the LM5 epitope and stronger detection of the LM2 epitope.

(B) Roots of FKBP12-expressing wild-type seedlings grown in the absence (left) and presence (right) of rapamycin. The presence of rapamycin led to a reduced labeling with LM5 and a stronger labeling in proximal parts with LM2.

Arrowheads, root apex. Bars = 0.3 mm.